5 Simple Statements About attune flow cytometer Explained
5 Simple Statements About attune flow cytometer Explained
Blog Article
Data-enriched characterization of mobile populations. Morphological data from photos can increase to your richness of flow cytometry knowledge. By way of example, the determine demonstrates an otherwise typical apoptosis assay employing Annexin V and PI, introducing mobile imaging to characterize cells in Every populace to reveal morphologically distinctive attributes. These insights couldn't have already been received from multiplex staining by yourself.
Whether you’re a beginner or a professional, investigate our sources to progress your exploration and experience in flow cytometry.
Mix acoustic concentrating know-how with higher-parameter Investigation for adaptable spectral unmixing and traditional workflows.
Discover new insights into your experimental data by visualizing populations working with combined fluorescence and morphology details. Our software package interface wizards empower downsampling, concatenation (merged files) and dimensionality equipment that happen to be crafted in to the computer software for an intuitive, streamlined data Evaluation encounter.
"An image isn't rather worthy of a thousand dots, but it really seriously helps you to see pictures. It is excellent to determine visuals that correlate with staining. By way of example, AnnexinV-positive cells which can be morphologically various than healthy cells, or CD14+ monocytes that happen to be larger sized plus much more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it is illuminating to find out the quantity of doublets slip through regular singlet gates, and excellent to verify the standing of unanticipated double-beneficial events in my Examination."
Pair your Attune flow cytometer with an autosampler for greater workflow effectiveness, and include robotic integration for maximum throughput.
Consistent graphic quality even at higher throughput Acoustic concentrating and a superior-pace digital camera Mix to image these T cells continuously at lower or high flow costs. Effortlessly regulate emphasis and camera configurations to satisfy experimental demands.
The Attune NxT cytometer is the responsible workhorse instrument your lab can depend on for steady and trustworthy effectiveness. The Attune CytPix design contains a significant-speed brightfield digicam and AI-driven image Examination program, with modern upgrades which includes device Discovering equipment and an intuitive person interface, enabling Increased information precision, precision, and more quickly insights into sample biology.
Maybe most significantly, these situations consist of cells of Plainly distinctive phenotypes which could lead on to incorrect conclusions relating to double optimistic occasions (specifically in rare populations).
Sound waves help precise cell alignment, leading to Fantastic data top quality and ~10X more rapidly sample flow charges.
We Earlier shown the strength of imaging Automobile-T/Ramos mobile interactions. Allow’s examine just the populace of greatest desire, the double constructive activities, To find out more. We are able to now use extended morphological parameters (here, circularity and skewness of depth) to even further examine the characteristics of those populations and refine gating on these occasions, escalating facts robustness.
Crafted-in dimensionality reduction resources improved resolution into populations. Fluorescently https://mediajx.com/story22135003/flow-cytometer-a-revolutionary-tool-for-cell-analysis gated populations again-gated on to UMAP plot to verify envisioned population resolution in lysed whole human blood. Consumer can view events from Each and every cluster for visual affirmation of cell phenotypes.
Versatile payment approach from Agilent Economic Answers permit you to have the essential instrumentation you'll need for fast job, or to grow your lab.
At the guts of each and every Attune Flow Cytometer lies our innovative acoustic focusing technological know-how, which makes use of sound waves to meticulously align cells in the flow mobile. This progressive strategy surpasses classic hydrodynamic techniques, resulting in more quickly processing speeds, exceptional data good quality, and enhanced sample throughput.
The Attune Xenith cytometer is a significant-parameter spectral analyzer, offering both equally spectral unmixing and standard flow capabilities for detailed and versatile mobile analysis.
The Attune CytPix Flow Cytometer permits people to visualise samples for the duration of acquisition, confirm the precision of gating strategies, and aid final results with responsible documentation for publication reasons.
Below, we clearly show that by gating approaches utilizing picture-centered quantitative parameters can distinguish interacting cells from coincident events to far more accurately assess interacting cells.